STEP ONE

TECHNICAL PRODUCT BULLETIN #B-43
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
ORIGINAL LISTING DATE: MARCH 12, 1992
REVISED LISTING DATE: MARCH 21, 1997
"STEP ONE"
(a/k/a B&S INDUSTRIAL)
LISTING CHANGES PENDING; VERIFICATION BY MANUFACTURER AND EPA REQUIRED

I. NAME, BRAND, OR TRADEMARK
STEP ONE Type of Product: Biological Additive

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
B & S Research, Inc. 4345 Highway 21 Embarrass, MN 55732 Phone: (218) 984-3757 Fax: (218) 984-3212 Email: [email protected] (Mr. H.W. Lashmett)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
B&S Research, Inc. 4345 Highway 21 Embarrass, MN 55732 Phone: (218) 984-3757 Fax: (218) 984-3212 Email: [email protected] (Mr. H.W. Lashmett) Bioremediation International 4345 Highway 21 Embarrass, MN 55732 Phone: (218) 984-3757 Fax: (218) 984-3212 Email: [email protected] Farm for Profit Research and Development 4345 Highway 21 Embarrass, MN 55732 Phone: (800) 232-7692 Fax: (218) 984-3212 Email: [email protected]

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS

B&S Research, Inc.
4345 Highway 21
Embarrass, MN 55732
Phone: (218) 984-3757
Fax: (218) 984-3212
Email: [email protected]
(Mr. H.W. Lashmett)

Bioremediation International
4345 Highway 21
Embarrass, MN 55732
Phone: (218) 984-3757
Fax: (218) 984-3212
Email: [email protected]

Farm for Profit Research
and Development
4345 Highway 21
Embarrass, MN 55732
Phone: (800) 232-7692
Fax: (218) 984-3212
Email: [email protected]

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: None 2. Ventilation: Normal ventilation is adequate 3. Skin and eye contact; protective clothing; treatment in case of contact: Avoid contact with skin, especially when there are open wounds. In case of skin contact, wash with soapy water. The use of protective gloves is recommended. Avoid contact with eyes. In case of eye contact, immediately flush eyes with plenty of water continuously for at least 15 minutes. Consult a physician. The use of protective goggles is recommended. Avoid inhalation and ingestion. It is recommended that workers wear dust mask and not eat or smoke while handling the product(s). 4.a. Maximum storage temperature: 90ºF 4.b. Minimum storage temperature: -30ºF 4.c. Optimum storage temperature range: 32ºF to 70ºF 4.d. Temperature phase separations and chemical changes: NA

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability:
None
2. Ventilation:
Normal ventilation is adequate
3. Skin and eye contact; protective clothing; treatment in case of contact:
Avoid contact with skin, especially when there are open wounds. In case of skin contact, wash with soapy water. The use of protective gloves is recommended. Avoid contact with eyes. In case of eye contact, immediately flush eyes with plenty of water continuously for at least 15 minutes. Consult a physician. The use of protective goggles is recommended. Avoid inhalation and ingestion. It is recommended that workers wear dust mask and not eat or smoke while handling the product(s).
4.a. Maximum storage temperature: 90ºF
4.b. Minimum storage temperature: -30ºF
4.c. Optimum storage temperature range: 32ºF to 70ºF
4.d. Temperature phase separations and chemical changes: NA

V. SHELF LIFE
Over 3 years

VI. RECOMMENDED APPLICATION PROCEDURE
1. Application Method: Components of the product STEP ONE (BC101) and STEP ONE (MSE2.5) are only sold together. B&S Research will make application recommendations based on contamination and ppm of contamination at time of purchase order and employ specified application methods (spray, plowing, agitation, etc.) appropriate for a particular situation. 2. Concentration/Application Rate: Nominally, the STEP ONE water mixture can be used on most hydrocarbons, including crude and refined petroleum products, pesticides, etc., on land, fresh water or ocean water. Laboratory tests also indicate effectiveness in destruction of PCB's. In certain circumstances, B&S Research, Inc. may require laboratory soil and water contamination tests before the product may be used (see VI 1. above).

VI. RECOMMENDED APPLICATION PROCEDURE

1. Application Method:
Components of the product STEP ONE (BC101) and STEP ONE (MSE2.5) are only sold together. B&S Research will make application recommendations based on contamination and ppm of contamination at time of purchase order and employ specified application methods (spray, plowing, agitation, etc.) appropriate for a particular situation.
2. Concentration/Application Rate:
Nominally, the STEP ONE water mixture can be used on most hydrocarbons, including crude and refined petroleum products, pesticides, etc., on land, fresh water or ocean water. Laboratory tests also indicate effectiveness in destruction of PCB's. In certain circumstances, B&S Research, Inc. may require laboratory soil and water contamination tests before the product may be used (see VI 1. above).

VII. TOXICITY AND EFFECTIVENESS

a. Toxicity:
NA

b. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:

Summary Data Table:

DAYS	PRODUCT 3 REPS/PROD	TOTAL MEAN ALKANES (ppm)	RED% 28 DAYS	TOTAL MEAN AROMATICS (ppm)	RED% 28 DAYS
0	CONTROL NUTRIENT STEP ONE	29959.5 29008.5 30477.8		5391.8 5163.0 5499.8
7	CONTROL NUTRIENT STEP ONE	33471.3 22723.7 24196.8		5720.9 5050.1 3110.2
28	CONTROL NUTRIENT STEP ONE	30997.4 1103.9 17059.4	0.0 % 96.19% 44.03%	5388.9 4582.4 2501.9	0.0 % 11.23% 54.51%

Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28
Control: 0%
Nutrient: 19%
Product (STEP ONE): 51%

VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of all microorganisms by species and percentages in the composition: CONFIDENTIAL 2. Optimum pH, temperature, and salinity ranges for use of the additive: pH: 6-8 Temperature: 70ºF to 90ºF Salinity: Fresh or salt (ocean < 110 ppm) water, land or dry surfaces 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: < 5 or > 9, (apply neutralizing agents -- lime or P205) Temperature: < 50ºF or > 135ºF Salinity: < 0 or > 110 ppm 4. Special nutrient requirements: None 5. Test results regarding the determination of the presence of the following: Salmonella: None found Fecal coliform: None found Shigella: None found Staphylococcus coagulase positive: None found Beta hemolytic Streptococci: None found

VIII. MICROBIOLOGICAL ANALYSIS

1. Listing of all microorganisms by species and percentages in the composition:
CONFIDENTIAL
2. Optimum pH, temperature, and salinity ranges for use of the additive:
pH: 6-8
Temperature: 70ºF to 90ºF
Salinity: Fresh or salt (ocean < 110 ppm) water, land or dry surfaces
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: < 5 or > 9, (apply neutralizing agents -- lime or P205)
Temperature: < 50ºF or > 135ºF
Salinity: < 0 or > 110 ppm
4. Special nutrient requirements:
None
5. Test results regarding the determination of the presence of the following:
Salmonella: None found
Fecal coliform: None found
Shigella: None found
Staphylococcus coagulase positive: None found
Beta hemolytic Streptococci: None found

IX. PHYSICAL PROPERTIES
1. Flash Point: > 212ºF 2. Pour Point: -7ºF 3. Viscosity: 2.8 cst at 50ºF 3 5 cst at 32ºF 5.9 cst at -4ºF 4. Specific Gravity: 1.03 at 59ºF 5. pH: 7.2 (1:2.5:80 BC101:MSE2.5:water mixture) 6. Chemical Name and Percentage by Weight of the Total Formulation: CONFIDENTIAL 7. Surface Active Agents: Noedal 8. Solvents: None 9. Additives: Phosphoric acid as P205 3% emulsifier < .5% by weight 10. Solubility: Infinite

IX. PHYSICAL PROPERTIES

1. Flash Point: > 212ºF
2. Pour Point: -7ºF
3. Viscosity:
2.8 cst at 50ºF
3 5 cst at 32ºF
5.9 cst at -4ºF
4. Specific Gravity: 1.03 at 59ºF
5. pH: 7.2 (1:2.5:80 BC101:MSE2.5:water mixture)
6. Chemical Name and Percentage by Weight of the Total Formulation:
CONFIDENTIAL
7. Surface Active Agents: Noedal
8. Solvents: None
9. Additives: Phosphoric acid as P205
3% emulsifier < .5% by weight
10. Solubility: Infinite

X. ANALYSIS FOR HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS

Compound	Concentration (ppm)
Arsenic	< 0.0200
Cadmium	< 0.0030
Chromium	0.0859
Copper	0.0177
Lead	0.0090
Mercury	0.0002
Nickel	< 0.0080
Zinc	0.0602
Cyanide	0.2830
Chlorinated Hydrocarbons	< 0.0050

STEP ONE

Emergency Response