Series 850 - Ecological Effects Test Guidelines

The final Ecological Effects Test Guidelines are generally intended to meet toxicity testing requirements for terrestrial and aquatic animals and plants under TSCA, FIFRA and FFDCA.

Supplemental Guidance

Guidance for Calculating Percent Survival in Seedling Emergence Studies

Part 158 Fish Bioconcentration Data Requirement: Guidance for Selection of Number of Treatment Concentrations (pdf) (234.22 KB, July 2020)

Final Guidance for Waiving Sub-Acute Avian Dietary Tests for Pesticide Registration and Supporting Retrospective Analysis (pdf) (1.06 MB)

Group A - Aquatic and Sediment-dwelling Fauna and Aquatic Microcosms

Group B - Terrestrial Wildlife

850.2000 - Background and Special Considerations- Tests with Terrestrial Wildlife (June 2012)
850.2100 - Avian Acute Oral Toxicity Test (June 2012)
850.2200 - Avian Dietary Toxicity Test (June 2012)
850.2300 - Avian Reproduction Test (June 2012)
850.2400 - Wild Mammal Toxicity Testing (June 2012)
850.2500 - Field Testing for Terrestrial Wildlife (June 2012)

Group C - Terrestrial Beneficial Insects, Invertebrates, and Soil and Wastewater Microorganisms

850.3000 - Background and Special Considerations- Tests with Terrestrial Beneficial Insects, Invertebrates and Microorganisms (June 2012)
850.3020 - Honey Bee Acute Contact Toxicity Test (June 2012)
850.3030 - Honey Bee Toxicity of Residues on Foliage (June 2012)
850.3040 - Field Testing for Pollinators (June 2012)
850.3100 - Earthworm Subchronic Toxicity Test (June 2012)
850.3200 - Soil Microbial Community Toxicity Test (June 2012)
850.3300 - Modified Activated Sludge, Respiration Inhibition Test (June 2012)

Group D - Terrestrial and Aquatic Plants, Cyanobacteria, and Terrestrial Soil Core Microcosm

850.4000 - Background and Special Considerations-Tests with Terrestrial and Aquatic Plants, Cyanobacteria, and Terrestrial Soil-Core Microcosms (June 2012)
850.4100 - Seedling Emergence and Seedling Growth (June 2012)
850.4150 - Vegetative Vigor (June 2012)
850.4230 - Early Seedling Growth Toxicity Test (June 2012)
850.4300 - Terrestrial Plants Field Study (June 2012)
850.4400 - Aquatic Plant Toxicity Test Using Lemna spp. (June 2012)
850.4450 - Aquatic Plants Field Study (June 2012)
850.4500 - Algal Toxicity (June 2012)
850.4550 - Cyanobacteria (Anabaena flos-aquae) Toxicity (June 2012)
850.4600 - Rhizobium-Legume Toxicity (June 2012)
850.4800 - Plant Uptake and Translocation Test (June 2012)
850.4900 - Terrestrial Soil-Core Microcosm Test (June 2012)

Group F - Field Test Data Reporting Guidelines

850.6100 - Environmental Chemistry Methods and Associated Independent Laboratory Validation (June 2012)